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KMID : 0624620090420010035
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BMB Reports
2009 Volume.42 No. 1 p.35 ~ p.40
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PKD2 interacts with Lck and regulates NFAT activity in T cells
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Li Qing
Sun Xiao-Qing
Wu Jun
Lin Zhi-Xin
Luo Ying
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Abstract
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Protein kinase D2 (PKD2) is a member of the PKD serine/threonine protein kinase family that has been implicated in the regulation of a variety of cellular processes including proliferation, survival, protein trafficking and immune response. In the present study, we report a novel interaction between PKD2 and Lck, a member of the Src tyrosine protein kinase family that is predominantly expressed in T cells. This interaction involved the C-terminal kinase domains of both PKD2 and Lck. Moreover, co-expression of Lck enhanced the tyrosine phosphorylation of PKD2 and increased its kinase activity. Finally, we report that PKD2 enhanced T cell receptor (TCR)-induced nuclear factor of T cell (NFAT) activity in Jurkat T cells. These results suggested that Lck regulated the activity of PKD2 by tyrosine phosphorylation, which in turn may have modulated the physiological functions of PKD2 during TCR-induced T cell activation.
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KEYWORD
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AP-1, Lck, NFAT, PKD2, TCR
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